ChIP

[invite807a92f5]

Bonjour,

quelqu'un connait-il la 1ère publi sur l'immunoprécipitation de chromatine (ChIP) ? celle qui présente la méthode

merci
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[invite8c0cc995]

Analysis of Chromatin Structure byin VivoFormaldehyde Cross-Linking

Valerio Orlando1, Helen Strutt and Renato Paro2
ZMBH, Center for Molecular Biology, University of Heidelberg, Im Neuenheimer Feld 282, 69120, Heidelberg, Germany

Abstract

Recent advances leave no doubt that higher order chromatin structures play a fundamental role in many developmentally important mechanisms of gene regulation. In particular analyses in genetic model systems like yeast andDrosophilauncovered novel proteins that are involved in the regulation of chromatin structures. Many of these proteins do not bind directly to DNA but interact in large multimeric complexes. To identify the DNA elements regulated by these multiprotein complexes, alternative approaches to the standard methods of DNA–protein analysis had to be devised. Here we present a method that preserves the architecture of the higher order chromatin structures by cross-linking cellsin vivowith formaldehyde. An immunoprecipitation strategy is then used to identify the DNA targets of chromosomal proteins of interest. This method can be applied to study the distribution of proteins at high resolution over extended chromosomal regions.

[invite807a92f5]

merci de ton aide. En fait, la méthode est assez récente.

Malheureusement, la publi n'est pas en accès libre. Est-ce que tu aurais la publi en entier par hasard?