Bonjour,
Je n'arrive pas a comprendre la technique du voltage clamp.
J'ai pu voir que cette technique permettais de fixer le potentiel de membrane a une valeur souhaité. Cependant je ne comprend pas comment, ni ce que cela nous apports.
J'ai trouvé un texte dessus (en anglais).
Cependant je ne comprend pas tout. Si quelqu'un peut bien m'expliquer, je lui en serais très reconnaissant !
Voici le texte :
"The voltage clamp is an experimental method used by electrophysiologists to measure the ion currents through the membranes of excitable cells, such as neurons, while holding the membrane voltage at a set level. A basic voltage clamp will iteratively measure the membrane potential, and then change the membrane potential (voltage) to a desired value by adding the necessary current. This "clamps" the cell membrane at a desired constant voltage, allowing the voltage clamp to record what currents are delivered. Because the currents applied to the cell must be equal to (and opposite in charge to) the current going across the cell membrane at the set voltage, the recorded currents indicate how the cell reacts to changes in membrane potential. Cell membranes of excitable cells contain many different kinds of ion channels, some of which are voltage-gated. The voltage clamp allows the membrane voltage to be manipulated independently of the ionic currents, allowing the current-voltage relationships of membrane channels to be studied. "
Texte tiré de Wikipedia
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