Bonjour,
Je suis en train de lire une publi mais je ne comprends pas grand-chose à cette partie des "Matériels et méthodes"
Donc, il s'agit de:
thick tassel dwarf1 encodes a putative maize ortholog of the Arabidopsis CLAVATA1 leucine-rich repeat receptor-like kinaseGenetics
The glassfingers (glf) mutation (now called td1-glf) was mapped using waxy translocations to chromosome 5S. Complementation crosses with td1-Ref (obtained from the Maize Genetics Cooperative Stock Center) confirmed allelism. Both td1-glf and td1-Ref were introgressed into B73 and W22 for phenotypic observations. Additional alleles (see Table 1) were obtained by crossing td1-Ref or td1-glf homozygotes as males onto Mutator stocks. Screens were carried out in Davis, California and in Illinois. td1-mum3 was identified in a F2 family of the Maize Gene Discovery Project (Lunde et al., 2003Go). A F2 family segregating for fea2, td1 double mutants was created by crossing individuals homozygous for fea2-O and td1-glf respectively, and selfing the resulting progeny. Both mutations were in the B73 background.
Development 132, 1235-1245 (2005)
Qu'est-ce que les "waxy translocations"? Et que veut dire "introgressed" en termes de manip? Enfin, qu'est-ce que "selfing the resulting progeny"?
Merci pour vos réponses!
Cordialement,
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