Bonjour,
j'aurais aimé savoir pourquoi, pour certaines études, le fond génétique BALB/c est privilégié (au lieu de C57BL/6 par ex)? Ce fond est très souvent utilisé en cancéro, immuno, mais pourquoi? A t il des susceptibilités particulières? Un génome particulier?
merci beaucoup pour vos réponses.
Alice
Alors tout d'abord, le fait que des études utilisent telle ou telle souche ne veut pas forcément dire que c'est la plus adaptée pour ce type de protocole, il peut s'agir de la disponibilité de la souche, ou d'une souche transgénique qui existe déjà sur ce fond là, du coup pas besoin de faire de backcross.
Ensuite, il y a effectivement des différences génétiques à l'origine d'un phénotype qui peut changer entre les souches. Sur le site du jackson lab, il y a énormément d'informations sur les caractéristiques des souches.
Pour les BALB/c, on peut lire par exemple :
ou encoreAppearance
albino
Related Genotype: A/A Tyrp1b/Tyrp1b Tyrc/Tyrc
Description
BALB/c mice are particularly well known for the production of plasmacytomas following injection with mineral oil forming the basis for the production of monoclonal antibodies. Although not all BALB/c substrains have been examined for plasmacytoma induction, substrains derived from the Andervont (An) lineage (which includes BALB/cByJ) typically are susceptible, while those descended from BALB/cJ are resistant (see: Potter M ,1985). Mammary tumor incidence is normally low but infection with mammary tumor virus by fostering to MMTV+ C3H mice dramatically increases tumor number and age of onset. BALB/c mice develop other cancers later in life including reticular neoplasms, primary lung tumors, and renal tumors. Rare spontaneous myoepitheliomas arising from myoepithelial cells of various exocrine glands have been observed in both BALB/cJ and BALB/cByJ substrains.
White et al. reported a variation in thioglycolate medium-induced peritoneal leukocyte recruitment in 4 analyzed strains. The response of total leukocyte recruitment, from greatest to least, was C57BL/6J>BALB/c>CD1>129X1/SvJ. Variations were also found in the timeline of response and cell types most impacted.
Dorso-ventral vaginal septa is observed in some BALB/cJ females, and may contribute to non-productive females in this strain. (Cunliffe-Beamer T, Lab Anim Sci, 1976)
assigned by genotype
Hld/Hld
either: BALB/cByJ or BALB/cJ
nervous system phenotype
abnormal dendrite morphology
abnormally positioned by being late-generated (MGI Ref ID J:12029)
cell bodies are located below the intrapyramidal mossy fiber layer (MGI Ref ID J:12029)
abnormal hippocampus morphology (MGI Ref ID J:13989)
abnormal hippocampal mossy fiber morphology (MGI Ref ID J:12029)
abnormal hippocampus CA3 region morphology
there is abnormal lamination of this cell layer resulting in superficial early generated neurons and deep late-generated neurons (MGI Ref ID J:12029)
abnormal hippocampus layer morphology
the anomaly is also seen in BALB/cByJ, F1 mice involving BALB sublines noted, and some CXB RI lines; C57BL/6J is considered wild-type for this feature (MGI Ref ID J:13989)
this is secondary to a defect in neuronal migration (MGI Ref ID J:13989)
ectopic hippocampus pyramidal cells
located below the intrapyramidal layer; some of the ectopic cells have short, fine-caliber dendritic branches arising near the points of contact of the intrapyramidal mossy fibers (MGI Ref ID J:12029)
abnormal neuronal migration (MGI Ref ID J:13989)
Research Applications
This mouse can be used to support research in many areas including:
Cancer Research
Increased Tumor Incidence
Mammary Gland Tumors: late onset
Research Tools
General Purpose
Neurobiology Research
Behavioral and Learning Defects
high anxiety
Immunology and Inflammation Research
Inflammation
Cancer Research
Increased Tumor Incidence
Mammary Gland Tumors
Cardiovascular Research
Diet-Induced Atherosclerosis
Relatively Resistant
Neurobiology Research
Neurodevelopmental Defects
callosal agenesis, incomplete penetrance
Research Tools
Cancer Research
monoclonal antibodies, myeloma and hybridoma production
