Bonjour,
Je décide de sortir de ma coquille Procaryote et de faire un tour chez les autres
Mais voici problème. J'ai trouvé cet article dans le Cancer Gene Therapy de cette semaine mais ma petite tête n'y pige rien Voici juste l'abstract, je n'ai pas encore l'article entier :
DNA immunization using constant-current electroporation affords long-term protection from autochthonous mammary carcinomas in cancer-prone transgenic mice
C Curcio, A S Khan, A Amici, M Spadaro, E Quaglino, F Cavallo, G Forni and R Draghia-Akli, Cancer Gene Therapy (2008) 15, 108–114; doi:10.1038/sj.cgt.7701106
A recently developed, adaptive constant-current electroporation technique was used to immunize mice with an intramuscular injection of plasmid coding for the extracellular and transmembrane domains of the product of the rat neu664V-E oncogene protein. In wild-type BALB/c mice, plasmid electroporation at lower current settings elicits higher antibody titers, a strong cytotoxic response and completely protects all mice vaccinated with 10, 25 and 50 mug of plasmid against a lethal challenge of rat neu+ carcinoma cells. BALB/c mice transgenic for the transforming rat neu664V-E (ErbB-2, Her-2/neu) oncogene (BALB-neuT664V-E) develop an invasive mammary gland carcinoma by 20 weeks of age. Remarkably, when transgenic BALB-neuT664V-E mice were vaccinated at a 10- week interval with 50 mug of plasmid with 0.2 A electroporation, mice remained tumor free for more than a year. A single administration of plasmid associated with electroporation was enough to markedly delay carcinogenesis progression in mice with multiple microscopic invasive carcinomas, and keep about 50% of mice tumor free at one year of age. Thus, vaccination using a clinically relevant dose of plasmid encoding the extracellular and transmembrane domains of the neu oncogene delivered by electroporation prevents long-term tumor formation. These improvements in the efficacy of this cancer vaccine regimen vastly increase its chances for clinical success.
Quelqu'un saurait m'expliquer avec des mots simples ce que c'est que la thérapie par courant continu? On injecte un plasmide recombinant continuellement dans le muscle de la souris, une espèce de transfusion plasmidique?
Merci pour vos réponses.
Cordialement,
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