Bonjour,
je souhaiterais savoir si qqn a déjà effectuer des analyses de peroxydes organiques par HPLC afin de savoir quelle méthode utiliser et peut-être quel solvant....
Pour les colonne j'ai presque trouvé mais si qqn avait un début de méthode ça m'aiderait bcp...
merci a tous
bonne journée
Bonjour Bubin;
J'ai un autre protocle expérimental sur les huiles, qui est une analyse chimique.
Salut
as-tu essayé de faire un peu de biblio ?
ta question est assez vague et la réponse dépend énormément du type de peroxyde que tu veux analyser
voilà quelques réponse que j'obtiens sur scirus.com
Xu X, van de Craats AM, Kok EM, de Bruyn PC
J Forensic Sci. 2004 Nov;49(6):1230-6.
Trace analysis of peroxide explosives by high performance liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry (HPLC-APCI-MS/MS) for forensic applications.
Saiz AI, Manrique GD, Fritz R.
J Agric Food Chem. 2001 Jan;49(1):98-102.
Determination of benzoyl peroxide and benzoic acid levels by HPLC during wheat flour bleaching process.
Freshly milled wheat flour has a pale yellow color due to its carotenoids content. Benzoyl peroxide is a bleaching agent typically used to give such flour a better appearance. This free-radical initiator promotes carotenoids oxidation, thereby producing less colored compounds, and benzoic acid is a main final product. Samples of wheat flour were treated with 150 ppm of benzoyl peroxide to begin a bleaching process, and then subjected to ethyl ether extraction at different intervals of time. Benzoyl peroxide and benzoic acid levels in these extracts were monitored by means of HPLC in individual experiences. The resulting concentration of benzoyl peroxide after 9 days of contact with the bleaching agent was 11 ppm, dropping afterward to nondetectable levels. A maximum value for benzoic acid of 16 ppm was found after 12 h of bleaching. Subsequently this level decreased continuously until reaching a residual value of 6 ppm after 3 months.
